The chemical component information of samples was analyzed by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS). The leaves and flosses of Artemisia argyi and A. stolonifera from different places, were distinguished by principal component analysis(PCA) and orthogonal partial least squares discriminant analysis(OPLS-DA). Then, compounds with significant differences(P<0.01) in abundance were screened out according to their VIP values and t-test. Eighteen components in all samples have been filtered and identified, including flavonoids and chlorogenic acids, the content of the 12 of which were measured by UPLC-UV, which are different in presence and content. Hispidulin in A. argyi is not detected in A. stolonifera. Schaftaside, isochlorogenic acid B, and isochlorogenic acid C are differential compounds of A. argyi and A. stolonifera leaves. Isochlorogenic acid A, isochlorogenic acid C and jaceosidin are differential compounds of A. argyi and A. stolonifera floss. There are significant differences in the contents of jaceosidin and schaftoside in the four famous A. argyi. In addition, the content of isochlorogenic acid A in wild A. stolonifera is higher than that in cultivated A. stolonifera. The results of the study successfully clarified the differences between A. stolonifera and A. argyi, and provided theoretical and data references for the further development and utilization of A. stolonifera.
Keywords: Artemisia argyi; Artemisia stolonifera; UPLC-Q-TOF-MS; chlorogenic acids; composition differences; flavonoids.