Influence of sanguinarine-based phytobiotic supplementation on post necrotic enteritis challenge recovery

Mashael R Aljumaah; Manal M Alkhulaifi; Riyadh S Aljumaah; Alaeldein M Abudabos; Abdulaziz A Abdullatif; Gamaleldin M Suliman; Mu'ath Q Al-Ghadi; Dragana Stanley

doi:10.1016/j.heliyon.2020.e05361

Influence of sanguinarine-based phytobiotic supplementation on post necrotic enteritis challenge recovery

Heliyon. 2020 Nov 2;6(11):e05361. doi: 10.1016/j.heliyon.2020.e05361. eCollection 2020 Nov.

Authors

Mashael R Aljumaah¹, Manal M Alkhulaifi¹, Riyadh S Aljumaah², Alaeldein M Abudabos², Abdulaziz A Abdullatif², Gamaleldin M Suliman², Mu'ath Q Al-Ghadi³, Dragana Stanley⁴

Affiliations

¹ Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia.
² Department of Animal Production, College of Food and Agriculture Sciences, King Saud University, Riyadh, Saudi Arabia.
³ Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.
⁴ Institute for Future Farming Systems, Central Queensland University, Rockhampton, Queensland, Australia.

Abstract

In the animal production industry, plant-derived antimicrobial phytobiotics are used as an alternative to antibiotics. Here we investigated the role sanguinarine-based phytobiotic in broiler recovery from Necrotic Enteritis (NE) infection. A total of 100 one-day-old broiler chicks (Ross 308) were randomly allocated to four treatments: negative control CTR (no challenge, no phytobiotic supplementation); positive control NE (NE challenged); phytobiotic SG (sanguinarine phytobiotic, 0.12 g/kg); and SG + NE, (sanguinarine phytobiotic, 0.12 g/kg and NE challenge). Sanguinarine-based phytobiotic supplementation caused significant changes between the groups in performance, livability and histological measurements, however, these changes were not significantly different between SG + NE and NE groups. Significant improvement was detected in NE lesion score of the duodenum and ileum of SG + NE birds compared to NE challenged birds at the end of the production cycle at 40 days old, indicating improved post-NE recovery with the addition of phytobiotic. Sanguinarine-based phytobiotic supplementation in NE challenged birds significantly compensated for a NE associated reduction of Firmicutes and an increase in Bacteroidetes. Functional profile of sanguinarine-based phytobiotic supplemented birds microbiota was distinct from CTR functional profile. NE challenge was associated with a significant increase in cecal propionic acid, while sanguinarine-based phytobiotic supplementation resulted in an increase in cecal acetic acid.

Keywords: Broiler; Food microbiology; Food safety; Infectious disease; Intestine; Microbiology; Necrotic enteritis; Nutrition; Phytobiotic; Sanguinarine.