Culture-based Methods for Studying the Bacterial Root Microbiome of Wheat

Rebekah J Robinson; Vanessa N Kavamura; Penny R Hirsch; Ian M Clark; Tim H Mauchline

doi:10.1007/978-1-0716-1040-4_6

Culture-based Methods for Studying the Bacterial Root Microbiome of Wheat

Methods Mol Biol. 2021:2232:53-60. doi: 10.1007/978-1-0716-1040-4_6.

Authors

Rebekah J Robinson¹, Vanessa N Kavamura¹, Penny R Hirsch¹, Ian M Clark¹, Tim H Mauchline²

Affiliations

¹ Department of Sustainable Agriculture Sciences, Rothamsted Research, Harpenden, Herts, UK.
² Department of Sustainable Agriculture Sciences, Rothamsted Research, Harpenden, Herts, UK. tim.mauchline@rothamsted.ac.uk.

PMID: 33161539
DOI: 10.1007/978-1-0716-1040-4_6

Abstract

Beneficial plant-microbe interactions are important and desirable for sustainable intensification of agriculture. Here, we describe methods to isolate microbes from the roots of field-grown wheat plants. This includes the rhizosphere and rhizoplane soil, as well as the root endosphere. We also describe a method to test for endosphere competence of putative endophytes.

Keywords: Endophyte; Endosphere; Microbiome; Rhizoplane; Rhizosphere; Root.

MeSH terms

Agriculture / methods*
Culture Techniques / methods*
Endophytes / genetics
Microbiota / genetics
Plant Roots / genetics
Plant Roots / microbiology*
Triticum / genetics
Triticum / microbiology*