Genetic diversity of N-fixing and plant growth-promoting bacterial community in different sugarcane genotypes, association habitat and phenological phase of the crop

Arch Microbiol. 2021 Apr;203(3):1089-1105. doi: 10.1007/s00203-020-02103-7. Epub 2020 Nov 8.

Abstract

This study aimed to evaluate the genetic diversity of bacterial community associated to different sugarcane genotypes, association habitat and phenological phase of the culture, as well as to isolate, to identify and to characterize your potential for plant growth-promoting. Root and rhizospheric soil samples from RB 92579 and RB 867515 varieties were collected at 120 and 300 days after regrowth (DAR). The diversity of bacterial was evaluated through of the 16S rRNA and nifH genes. We found greater genetic diversity in the root endophytic habitat at 120 DAR. We identify the genera Burkholderia sp., Pantoea sp., Erwinia sp., Stenotrophomonas sp., Enterobacter sp. and Pseudomonas sp. The genera Bacillus sp. and Dyella sp. were only identified in the variety RB 92579. We found indices above 50% for biological nitrogen fixation, production of indole acetic acid and phosphate solubilization, showing that the use of these bacteria in biotechnological products is very promising.

Keywords: BOX-PCR; Biological N fixation; DGGE; Phosphate solubilization; Production of indole acetic acid.

MeSH terms

  • Bacteria / genetics*
  • Ecosystem*
  • Genetic Variation*
  • Genotype
  • Indoleacetic Acids
  • Nitrogen Fixation / physiology
  • Plant Development / physiology
  • Plant Roots / microbiology*
  • RNA, Ribosomal, 16S / genetics
  • Rhizosphere
  • Saccharum / microbiology*

Substances

  • Indoleacetic Acids
  • RNA, Ribosomal, 16S
  • indoleacetic acid