Multiple Imaging Modalities for Cell-Cell Communication via Calcium Mobilizations in Corneal Epithelial Cells

Yoonjoo K Lee; Kristen L Segars; Vickery Trinkaus-Randall

doi:10.1007/7651_2020_329

Multiple Imaging Modalities for Cell-Cell Communication via Calcium Mobilizations in Corneal Epithelial Cells

Methods Mol Biol. 2021:2346:11-20. doi: 10.1007/7651_2020_329.

Authors

Yoonjoo K Lee¹, Kristen L Segars¹, Vickery Trinkaus-Randall²

Affiliations

¹ Departments of Pharmacology and Biochemistry, Boston University School of Medicine, Boston, MA, USA.
² Departments of Pharmacology and Biochemistry, Boston University School of Medicine, Boston, MA, USA. vickery@bu.edu.

Abstract

Chemical indicators are used to study calcium signaling events in the context of live cell imaging. Fluo-3 AM, Fluo-4 AM, and Cal-520 AM are three commonly used fluorescent indicators derived from the calcium chelator BAPTA. Here we describe sample protocols that detail how these indicators are used in in vitro and ex vivo experiments to analyze the role of calcium mobilizations in cell-cell communication and coordinated cellular motility in the context of wound healing.

Keywords: Calcium mobilization; Cell-cell communication; Confocal imaging; Ex vivo in situ analysis; Image analysis; Live cell imaging.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Calcium / metabolism*
Cell Communication
Epithelium, Corneal / cytology
Epithelium, Corneal / metabolism*
Humans

Substances

Calcium

Abstract

Publication types

MeSH terms

Substances

Grants and funding