Identification of milk adulteration by a sensor array based on cationic polymer induced aggregation of a perylene probe

Ling Zhang; Jiaze Hou; Huipeng Zhou; Muhammad Azhar Hayat Nawaz; Yongxin Li; Hui Huang; Cong Yu

doi:10.1016/j.foodchem.2020.128492

Identification of milk adulteration by a sensor array based on cationic polymer induced aggregation of a perylene probe

Food Chem. 2021 May 1:343:128492. doi: 10.1016/j.foodchem.2020.128492. Epub 2020 Oct 28.

Authors

Ling Zhang¹, Jiaze Hou², Huipeng Zhou³, Muhammad Azhar Hayat Nawaz⁴, Yongxin Li⁵, Hui Huang⁶, Cong Yu⁷

Affiliations

¹ College of Food Science and Engineering, Jilin University, Changchun 130025, PR China.
² College of Food Science and Engineering, Jilin University, Changchun 130025, PR China; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
³ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
⁴ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China; University of Science and Technology of China, Hefei 230026, PR China.
⁵ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China; College of New Energy and Environment, Jilin University, Changchun 130021, PR China. Electronic address: liyongxin@jlu.edu.cn.
⁶ College of Food Science and Engineering, Jilin University, Changchun 130025, PR China. Electronic address: huanghui@jlu.edu.cn.
⁷ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China; University of Science and Technology of China, Hefei 230026, PR China. Electronic address: congyu@ciac.ac.cn.

PMID: 33158685
DOI: 10.1016/j.foodchem.2020.128492

Abstract

A novel fluorescence sensor array based on cationic polymer induced self-assembly of a perylene probe is developed. Cationic polymer induced aggregation of the carboxyl modified negatively charged perylene probe, and resulted in large quenching of monomer emission and generation of excimer emission. Upon the addition of negatively charged protein, monomer fluorescence restored with a decrease in excimer fluorescence. Based on these observations, we developed a six-channel sensor array to discriminate five main proteins in milk. In addition, we successfully identified pure milk out of different drinks using the developed sensor array since different drinks contained distinct species and contents of proteins. Furthermore, the sensor array exhibited excellent performance to discriminate milk adulterated by different concentrations of adulterants with 100% accuracy of cross validation. The analysis results also presented excellent linear correlation of adulterants contents and thus the developed sensor array shows great potential for quantitative detection of milk adulteration.

Keywords: Cationic polymer; Fluorescence; Milk adulteration; Milk protein; Perylene probe; Sensor array.

MeSH terms

Animals
Food Analysis / instrumentation*
Food Quality
Fraud / prevention & control
Milk / chemistry*
Perylene / chemistry*
Polymers / chemistry*
Spectrometry, Fluorescence

Substances

Polymers
Perylene