Abstract
It is well recognized that isolated cardiac muscle cells beat in a periodic manner. Recently, evidence indicates that other, non-muscle cells, also perform periodic motions that are either imperceptible under conventional lab microscope lens or practically not easily amenable for analysis of oscillation amplitude, frequency, phase of movement and its direction. Here, we create a real-time video analysis tool to visually magnify and explore sub-micron rhythmic movements performed by biological cells and the induced movements in their surroundings. Using this tool, we suggest that fibroblast cells perform small fluctuating movements with a dominant frequency that is dependent on their surrounding substrate and its stiffness.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells
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Animals
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Cell Movement / physiology*
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Image Processing, Computer-Assisted / instrumentation
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Image Processing, Computer-Assisted / methods*
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Intravital Microscopy / instrumentation
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Intravital Microscopy / methods*
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Mice
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Microscopy, Video / instrumentation
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Microscopy, Video / methods*
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Time-Lapse Imaging / instrumentation
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Time-Lapse Imaging / methods*
Grants and funding
This project was supported by the Zimin Institute for Engineering Solutions Advancing Better Lives at Tel-Aviv University, Israel (
http://tau.zimininstitutes.org/), given to the last two authors (N.K. and A.L). H.W. acknowledges support from the Israel Science Foundation (1738/17;
https://www.isf.org.il/#/) and from the Rappaport Institute (
http://www.rappaport.org.il/Home). H.W. is an incumbent of the David and Inez Myers Career Advancement Chair in Life Sciences. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.