Status of the application of exogenous enzyme technology for the development of natural plant resources

Bioprocess Biosyst Eng. 2021 Mar;44(3):429-442. doi: 10.1007/s00449-020-02463-w. Epub 2020 Nov 4.

Abstract

Exogenous enzymes are extraneous enzymes that are not intrinsic to the subject. The exogenous enzyme industry has been rapidly developing recently. Successful application of recombinant DNA amplification, high-efficiency expression, and immobilization technology to genetically engineered bacteria provides a rich source of enzymes. Amylase, cellulase, protease, pectinase, glycosidase, tannase, and polyphenol oxidase are among the most widely used such enzymes. Currently, the application of exogenous enzyme technology in the development of natural plant resources mainly focuses on improving the taste and flavor of the product, enriching the active ingredient contents, deriving and transforming the structure of a chosen compound, and enhancing the biological activity and utilization of the functional ingredient. In this review, we discuss the application status of exogenous enzyme technology for the development of natural plant resources using typical natural active ingredients from plant, such as resveratrol, steviosides, catechins, mogrosides, and ginsenosides, as examples, to provide basis for further exploitation and utilization of exogenous enzyme technology.

Keywords: Active ingredients; Enzymatic assisted extraction; Exogenous enzyme technology; Immobilized enzyme technology; Natural product resources.

Publication types

  • Review

MeSH terms

  • Carboxylic Ester Hydrolases / chemistry*
  • Cellulase / chemistry*
  • Enzymes, Immobilized / chemistry*
  • Plants / chemistry*
  • Polygalacturonase / chemistry*

Substances

  • Enzymes, Immobilized
  • Carboxylic Ester Hydrolases
  • tannase
  • Polygalacturonase
  • Cellulase