New nanocarried phenobarbital formulation: Maintains better control of pentylenetetrazole-Induced seizures

Lorena Cristina Nunes de Almeida; Beatriz de Andrade Marques; Rafaela Laranjeira Silva; Akira Otake Hamoy; Vanessa Jóia de Mello; Rosivaldo Dos Santos Borges; Frank Sales Nunes Brito; Elson Longo; Marcos Anicete-Santos; Moisés Hamoy

doi:10.1016/j.btre.2020.e00539

New nanocarried phenobarbital formulation: Maintains better control of pentylenetetrazole-Induced seizures

Biotechnol Rep (Amst). 2020 Oct 9:28:e00539. doi: 10.1016/j.btre.2020.e00539. eCollection 2020 Dec.

Affiliations

¹ Laboratory of Pharmacology and Toxicology of Natural Products, Institute of Biological Sciences, Federal University of Pará, Belém, Brazil.
² NESBio, College of Pharmacy, Health Sciences Institute, Federal University of Pará, Belém, Pará, Brazil.
³ Nanobiotechnology Laboratory, Institute of Biological Sciences, Federal University of Pará, P.O. Box 479, 66075-110, Belém, Pará, Brazil.
⁴ Functional Materials Development Center (CDMF) - Federal University of São Carlos, Washington Luis Km 235, P.O. Box 676, 13565-905, São Carlos, São Paulo, Brazil.

Abstract

This study aims to evaluate the efficacy of slow release phenobarbital in the control of convulsions triggered by pentylenetetrazole (PTZ), verifying the time of permanence in the anticonvulsant effect through behavior and electroencephalographic records. A total of 162 male Wistar rats weighing between 100 and 120 g were divided into two groups, one for behavior analysis (n = 90) and biochemistry, and another for the acquisition of electrocorticographic record (n = 72). Hepatic enzymes were measured by obtaining a blood sample from the animals studied by means of a biochemical analysis. The procedures for electrode implant and electrocorticographic recordings were performed. The intercalation of phenobarbital in layered double hydroxide (LDH) nanocarrier allowed us to evaluate a new slow release pharmaceutical formulation based on methodologies that have proven longer residence time and lower side effects. This study demonstrates that phenobarbital can be a new perspective pharmaceutical formulation.

Keywords: Anticonvulsant; Drug delivery systems; Layered double hydroxide; Nanocarrier; Phenobarbital.