Improving nisin production by encapsulated Lactococcus lactis with starch/carboxymethyl cellulose edible films

Wenting Lan; Rong Zhang; Tengteng Ji; Dur E Sameen; Saeed Ahmed; Wen Qin; Jianwu Dai; Li He; Yaowen Liu

doi:10.1016/j.carbpol.2020.117062

Improving nisin production by encapsulated Lactococcus lactis with starch/carboxymethyl cellulose edible films

Carbohydr Polym. 2021 Jan 1:251:117062. doi: 10.1016/j.carbpol.2020.117062. Epub 2020 Sep 9.

Authors

Wenting Lan¹, Rong Zhang¹, Tengteng Ji¹, Dur E Sameen¹, Saeed Ahmed¹, Wen Qin¹, Jianwu Dai², Li He³, Yaowen Liu⁴

Affiliations

¹ College of Food Science, Sichuan Agricultural University, Yaan, 625014, China.
² College of Mechanical and Electrical Engineering, Sichuan Agricultural University, Ya'an, 625014, China.
³ College of Food Science, Sichuan Agricultural University, Yaan, 625014, China. Electronic address: helifood@163.com.
⁴ College of Food Science, Sichuan Agricultural University, Yaan, 625014, China; California Nano Systems Institute, University of California, Los Angeles, CA, 90095, USA. Electronic address: lyw@my.swjtu.edu.cn.

PMID: 33142614
DOI: 10.1016/j.carbpol.2020.117062

Abstract

In this study, Lactococcus lactis was embedded in a film of corn starch (NS) and carboxymethyl cellulose (CMC) prepared using a casting method. At a CMC:NS ratio of 5:5, the composite film had the best comprehensive properties. Scanning electron microscopy images clearly showed that L. lactis was effectively embedded. The film with 1.5 % L. lactis showed the best performance and the lowest water vapor transmission rate (5.54 × 10^-11 g/m s Pa. In addition, the edible film retained a viable count of 5.64 log CFU/g of L. lactis when stored at 4 °C for 30 days. The composite film with 1.5 % L. lactis showed the highest release of nisin (3.35 mg/mL) and good antibacterial activity against Staphylococcus aureus (53.53 %) after 8 days. Therefore, this edible film is a viable alternative antimicrobial strategy for the active packaging of foods containing low moisture content.

Keywords: Carboxymethyl cellulose; Edible film; Lactococcus lactis; Starch.

MeSH terms

Anti-Bacterial Agents / pharmacology
Carboxymethylcellulose Sodium / chemistry*
Edible Films / standards*
Food Packaging / methods*
Lactococcus lactis / chemistry*
Nisin / biosynthesis
Nisin / pharmacology*
Staphylococcus aureus / drug effects*
Staphylococcus aureus / growth & development
Starch / chemistry*

Substances

Anti-Bacterial Agents
Nisin
Starch
Carboxymethylcellulose Sodium