The purpose of this study was to investigate the effect of low-magnitude vibration on osteogenesis of osteoblasts in ovariectomized rats with osteoporosis via estrogen receptor α(ERα). The mRNA expression of osteogenic markers were examined with qRT-PCR, based on which the optimal vibration parameter for promoting osteogenesis was determined (45 Hz × 0.9 g, g = 9.8 m/s2). Then we loaded the optimal vibration parameter on the osteoblasts of ovariectomized rats with osteoporosis. The protein expression of osteogenic markers and ERα were detected with Western blot; the distribution of ERα was examined with immunofluorescence technique. Finally, through inhibiting the expression of ERα with estrogen receptor inhibitor ICI182780, the protein and mRNA expression of osteogenic markers were examined. First, the results showed that low-magnitude vibration could promote the expression of osteogenic markers and ERα in osteoblasts of ovariectomized rats with osteoporosis (P < 0.05), and make ERα transfer to the nucleus. On the other hand, the results also showed that after inhibiting the expression of ERα in osteoblasts of ovariectomized rats with osteoporosis, the protein and mRNA expression of osteogenic marker were decreased (P < 0.05). In our study, low-magnitude vibration played an important role in the osteogenesis of osteoblasts in ovariectomized rats with osteoporosis through increasing the expression and causing translocation of ERα. Furthermore, it provides a theoretical basis for the application of low-magnitude vibration in the prevention and treatment of postmenopausal osteoporosis.
为探讨低强度振动是否能经由雌激素受体 α（ERα）促进去卵巢骨质疏松症大鼠成骨细胞的骨形成，本研究通过采用实时荧光定量聚合酶链式反应（qRT-PCR）技术检测骨形成标志物 mRNA 表达量的变化，筛选出促进去卵巢骨质疏松症大鼠成骨细胞骨形成的最佳振动参数（45 Hz × 0.9 g，g 为重力加速度，g = 9.8 m/s2）。随后，对成骨细胞施加 45 Hz × 0.9 g 的低强度振动，采用免疫印迹（Western blot）实验检测骨形成标志物和 ERα 的表达；并用免疫荧光技术检测 ERα 的表达与分布。最后采用雌激素受体抑制剂 ICI182780，抑制 ERα 的表达，观察低强度振动下成骨细胞骨形成标志物的变化。结果显示，低强度振动可促进去卵巢骨质疏松症大鼠成骨细胞骨形成标志物和 ERα 的表达（P < 0.05），并使 ERα 向细胞核内转移；而当抑制 ERα 的表达后，低强度振动促进成骨细胞骨形成的作用减弱。以上结果说明，低强度振动可通过上调去卵巢骨质疏松症大鼠成骨细胞 ERα 的表达和促进 ERα 的胞核转移的方式，增强去卵巢骨质疏松症大鼠成骨细胞的骨形成功能，进而为低强度振动应用于临床绝经后骨质疏松症的防治提供理论依据。.
Keywords: estrogen receptor α; low-magnitude vibration; osteoblasts; ovariectomized osteoporosis.