Effect of a Neuropilin-1-Derived Virus Receptor Trap on Enterovirus A71 Infection In Vitro

Hsiang-Ching Wang; Peng-Nien Huang; Hui-Chen Hung; Sung-Nien Tseng; Chia-Chia Chang; Ya-Ru Tsai; Yun-Ming Wang; Shin-Ru Shih; John Tsu-An Hsu

doi:10.1128/AAC.00695-20

Effect of a Neuropilin-1-Derived Virus Receptor Trap on Enterovirus A71 Infection In Vitro

Antimicrob Agents Chemother. 2020 Dec 16;65(1):e00695-20. doi: 10.1128/AAC.00695-20. Print 2020 Dec 16.

Authors

Hsiang-Ching Wang^#^{1

2}, Peng-Nien Huang^#^{3

4}, Hui-Chen Hung^#⁵, Sung-Nien Tseng³, Chia-Chia Chang⁶, Ya-Ru Tsai⁵, Yun-Ming Wang⁷, Shin-Ru Shih^{3

8}, John Tsu-An Hsu^{9

5}

Affiliations

¹ Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan.
² Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, Hsinchu, Taiwan.
³ Research Center for Emerging Viral Infections, Chang Gung University, Taoyuan, Taiwan.
⁴ Division of Infectious Diseases, Department of Pediatrics, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan.
⁵ Institute of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli, Taiwan.
⁶ Animal Drugs Inspection Branch, Animal Health Research Institute, Council of Agriculture, Miaoli County, Taiwan.
⁷ Department of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, Center For Intelligent Drug Systems and Smart Bio-devices (IDS2B), National Chiao Tung University, Hsinchu, Taiwan.
⁸ Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan.
⁹ Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan tsuanhsu@nhri.edu.tw.

^# Contributed equally.

Abstract

We discovered that neuropilin 1 (NRP1) is a new receptor candidate to mediate enterovirus A71 (EVA71) into cells. In the engineered form as a decoy receptor, NRP1 was able to recognize and neutralize EVA71 but not enterovirus D68 or coxsackievirus B3 (CVB3). NRP1 recognizes EVA71 through a novel domain on the VP3 capsid protein. The principle in the design, engineering, and refinement of the NRP1-based decoy receptor described in this study represents a general and well-suited antiviral strategy.

Keywords: antiviral research; decoy receptor; enterovirus A71; neuropilin 1; virus receptor trap.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Enterovirus A, Human* / genetics
Enterovirus Infections*
Enterovirus*
Humans
Neuropilin-1 / genetics
Receptors, Virus / genetics

Substances

Receptors, Virus
Neuropilin-1