Bioactive components, antioxidant and antimicrobial activities of Paeonia rockii fruit during development

Abstract

In last ten years, much attention focused on tree peony fruit (TPF) for edible oil production despite other potential utilization. The present study identified and quantified 29 bioactive components by liquid chromatography-electrospray ionization-triple quadrupole-mass spectrometry (LC-ESI-QqQ-MS) targeted approach during the development of TPF. Trans-resveratrol, benzoic acid, luteolin, and methyl gallate were selected as predominant chemical markers between seeds and pods through principal component analysis (PCA) and orthogonal partial least square-discriminant analysis (OPLS-DA). Extremely high levels of paeoniflorin (1893 mg/100 g) and trans-resveratrol (1793 mg/100 g) were observed at stage 2 (S2) and S6 in seeds, respectively. Antioxidant activities determined by ABTS^+•, DPPH^•, and FRAP assays showed significant correlations with total phenolic content (TPC) and total flavonoid content (TFC). The strongest antibacterial effects of pod and seed against Staphylococcus aureus and Proteus vulgaris occurred at initial stages and maturation stages. TPF could be a potential source of bioactive compounds with functional properties.

Keywords: Albiflorin (PubChem CID: 24868421); Antibacterial activity; Antioxidant activity; Benzoic acid (PubChem CID: 243); LC-ESI-QqQ-MS; Luteolin (PubChem CID: 5280445); Methyl gallate (PubChem CID: 7428); Monoterpene glycosides; OPLS-DA; PCA; Paeonia rockii fruit; Paeoniflorin (PubChem CID: 442534); Phenolic compounds; Trans-resveratrol (PubChem CID: 445154).