Immune duration of a recombinant PRRSV vaccine expressing E2 of CSFV

Fei Gao; Yifeng Jiang; Guoxin Li; Yujiao Zhang; Kuan Zhao; Haojie Zhu; Liwei Li; Lingxue Yu; Hao Zheng; Yanjun Zhou; Wu Tong; Guangzhi Tong

doi:10.1016/j.vaccine.2020.10.057

Immune duration of a recombinant PRRSV vaccine expressing E2 of CSFV

Vaccine. 2020 Nov 25;38(50):7956-7962. doi: 10.1016/j.vaccine.2020.10.057. Epub 2020 Oct 31.

Authors

Fei Gao¹, Yifeng Jiang¹, Guoxin Li², Yujiao Zhang², Kuan Zhao², Haojie Zhu², Liwei Li², Lingxue Yu², Hao Zheng¹, Yanjun Zhou¹, Wu Tong¹, Guangzhi Tong³

Affiliations

¹ Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou 225009, PR China.
² Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
³ Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou 225009, PR China. Electronic address: gztong@shvri.ac.cn.

PMID: 33131934
DOI: 10.1016/j.vaccine.2020.10.057

Abstract

Classical swine fever virus (CSFV) and Porcine reproductive and respiratory syndrome virus (PRRSV) are both important pathogens which seriously harm the economic swine industry worldwide. We have previously demonstrated that rPRRSV-E2 is a promising live, virus-vectored vaccine that provides 100% protection against highly pathogenic PRRSV (HP-PRRSV) and CSFV. Here, we evaluated the duration of immunity (DOI) of the vaccine strain, rPRRSV-E2. Vaccine or cell culture medium was administered to piglets at 4 weeks of age. All immunized piglets developed high levels of antibodies, which could maintain for up to 23 weeks, against PRRSV and CSFV. All immunized pigs were well protected from the challenge of HP-PRRSV or CSFV at 20 weeks and 24 weeks post vaccination. The vaccine protection rate was still 100% at 24 weeks after immunization. The immune efficacy results showed that the immune duration of rPRRSV-E2 could be up to 5 months.

Keywords: Duration of immunity; Lung lesion; Viral load; rPRRSV-E2.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Viral
Classical Swine Fever Virus* / genetics
Classical Swine Fever* / prevention & control
Porcine Reproductive and Respiratory Syndrome* / prevention & control
Porcine respiratory and reproductive syndrome virus* / genetics
Swine
Viral Vaccines* / genetics

Substances

Antibodies, Viral
Viral Vaccines