Experimental self-etching resin infiltrants on the treatment of simulated carious white spot lesions

Linda Wang; Maria Cristina Carvalho de Almendra Freitas; Anuradha Prakki; Victor Mosquim; Alejandra Hortencia Miranda González; Daniela Rios; Heitor Marques Honório

doi:10.1016/j.jmbbm.2020.104146

Experimental self-etching resin infiltrants on the treatment of simulated carious white spot lesions

J Mech Behav Biomed Mater. 2021 Jan:113:104146. doi: 10.1016/j.jmbbm.2020.104146. Epub 2020 Oct 18.

Authors

Linda Wang¹, Maria Cristina Carvalho de Almendra Freitas², Anuradha Prakki³, Victor Mosquim⁴, Alejandra Hortencia Miranda González⁵, Daniela Rios⁶, Heitor Marques Honório⁶

Affiliations

¹ Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru-SP, Brazil. Electronic address: wang.linda@usp.br.
² Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru-SP, Brazil; DeVry FACID, DeVry Education Group, Teresina-PI, Brazil.
³ Faculty of Dentistry, University of Toronto, Restorative Department, Toronto-ON, Canada.
⁴ Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru-SP, Brazil.
⁵ Department of Restorative Dentistry, School of Dentistry, University of North Paraná, Londrina-PR, Brazil.
⁶ Department of Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo, Bauru-SP, Brazil.

PMID: 33125956
DOI: 10.1016/j.jmbbm.2020.104146

Abstract

Objectives: To evaluate the penetration depth (μm) of experimental resin infiltrants containing different percentages of triethylene glycol dimethacrylate (TEGDMA) and phosphoric acid 2-hydroxyethyl methacrylate ester (PAM) in artificial carious white spot lesions (WSL).

Methods: WSL were produced in 65 bovine flat enamel specimens by pH cycling protocol, which were treated with either Icon (control) or experimental acidic infiltrants based on different percentages of TEGDMA and PAM monomers (acidic), and their association or not with previous acid-etching with phosphoric acid. Ten readings using Confocal Laser Scanning Microscopy were conducted on each specimen and the penetration depth was calculated from the surface until the deepest point with the fluorescent dye Rhodamine B (0.02 mg/mL). The pH and the viscosity of the experimental infiltrants were also tested. Data were statistically analyzed with two-way ANOVA and Tukey tests (α < 0.05).

Results: The material factor and the interaction material*acid-etching were statistically significant. The lowest penetration depth was observed for the samples treated with the commercial infiltrant after etching with 15% hydrochloric acid. When specimens were pre-treated with PA, highest penetration was seen for specimens treated with 100% TEGDMA, which differed from all other groups. The lowest penetration was seen for those treated with 50:50 TEGDMA:PAM infiltrants. When specimens were not previously etched, highest penetration was seen for Icon, which differed only from those treated with 25% TEGDMA 75% PAM, where the lowest values were seen. The values of viscosity increased and the pH decreased with the addition of PAM in the infiltrant formulations.

Conclusion: the association between TEGDMA and PAM seems to allow similar infiltration depth reached by Icon infiltrant without acid etching the enamel surface.

Keywords: Caries; Dental enamel; Infiltrant resin; Permeability.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Composite Resins
Dental Caries* / drug therapy
Methacrylates*
Viscosity

Substances

Composite Resins
Methacrylates