Efficient Isolation and Phenotypic Profiling of Circulating Hepatocellular Carcinoma Cells via a Combinatorial-Antibody-Functionalized Microfluidic Synergetic-Chip

Lin Zhu; Huibin Lin; Shuang Wan; Xiaofeng Chen; Lingling Wu; Zhi Zhu; Yanling Song; Bin Hu; Chaoyong Yang

doi:10.1021/acs.analchem.0c03936

Efficient Isolation and Phenotypic Profiling of Circulating Hepatocellular Carcinoma Cells via a Combinatorial-Antibody-Functionalized Microfluidic Synergetic-Chip

Anal Chem. 2020 Nov 17;92(22):15229-15235. doi: 10.1021/acs.analchem.0c03936. Epub 2020 Oct 30.

Authors

Lin Zhu¹, Huibin Lin², Shuang Wan¹, Xiaofeng Chen¹, Lingling Wu², Zhi Zhu¹, Yanling Song¹, Bin Hu³, Chaoyong Yang^{1

2}

Affiliations

¹ The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
² Institute of Molecular Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
³ Clinical Laboratory of Oncology, Xiamen Cancer Center, School of Medicine, The First Affiliated Hospital of Xiamen University, Xiamen 361003, China.

PMID: 33124804
DOI: 10.1021/acs.analchem.0c03936

Abstract

As a malignant disease that seriously threatens human health, hepatocellular carcinoma (HCC) lacks effective early screening and prognostic assessment methods. Herein, we developed a method for efficient capture and multiphenotype analysis of circulating tumor cells (CTCs) of hepatocellular carcinoma. The anti-ASGPR antibody and the anti-EpCAM antibody were modified in parallel on a deterministic lateral displacement (DLD)-patterned microfluidic Synergetic-Chip to enhance capture efficiency by a complementary effect. CTCs were detected in 45 out of 45 (100%) HCC patients, with a sensitivity and specificity of 97.8 and 100%, respectively. Patients with more total CTCs and nonepithelial CTCs were in later stages of HCC and had more malignant progression. This strategy proposes a feasible approach for early diagnosis and prognosis of hepatocellular carcinoma.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Immobilized / chemistry*
Antibodies, Immobilized / immunology
Carcinoma, Hepatocellular / pathology*
Cell Line, Tumor
Cell Separation / instrumentation*
Epithelial Cell Adhesion Molecule / immunology
Humans
Lab-On-A-Chip Devices*
Liver Neoplasms / pathology*
Neoplastic Cells, Circulating / pathology*
Phenotype*

Substances

Antibodies, Immobilized
Epithelial Cell Adhesion Molecule