Glioma cells eradication by photoexcitation of bioengineered molybdenum trioxide nanoparticles synthesized by wet chemical and microwave route: Dose dependent photosensitizer bioactivity

Abstract

Here, we surveyed the usage of MoO₃ nanostructure in role of a photosensitizer to eradicate glioma cells. This is the first endeavor upon survey of usage of nanostructured MoO₃ to treat glioma in vitro. Here, we offer a simple way for preparation of bioactive MoO₃ nanostructure via two different routes; wet chemical and microwave. The influence of diverse experimental factors like various alcoholic solvents and presence of capping agent was investigated on the final properties of synthesized products. Dimension and morphology of inorganic molybdenum trioxide nanostructures checked with TEM, HRTEM and also SEM images. Moreover, the cytotoxicity effect of optimized MoO₃ nanoparticles was investigated on T98 and A172 cell lines. Both T98 and A172 cell lines indicated dose-dependent manner in the presence of increasing concentration of MoO₃ nanostructures, but T98 cells were less sensitive to MoO₃ in comparison with A172. Anti-glioma role of MoO₃ nanostructures excited with the aid of UVC illumination studied in vitro as well. By studying the UV exposure lonely, it is evident that UV effects on cell viability about 50% in both cell lines after 24 h. Interestingly, by combining nanostructured MoO₃ with UVC illumination, decrement in the proliferation value could be remarkably occurred in comparison with controls. The outcomes denote that the photodynamic therapy with the help of nanostructured MoO₃ may be beneficial to treat glioma.

Keywords: Cytotoxicity; Electron microscopy; Molybdenum trioxide; Nanostructure; UV exposure.