RNAi-based antiviral immunity of shrimp

Abstract

As a kind of important economic marine crustaceans in aquaculture, shrimp can be infected by more than 20 viruses. To fight against the virus invasion, shrimp have developed the innate immunity, including RNA interference (RNAi). RNAi, mediated by short interfering RNAs (siRNAs) or microRNAs (miRNAs), plays important roles in virus-host interactions. At present, RNAi is considered to be an efficient antiviral response of shrimp. The siRNA-based RNAi, first recognized as an antiviral response of animals to resist RNA viruses, has emerged in animals as an efficient antiviral strategy against the invasion of DNA viruses and RNA viruses. In shrimp, as well as in other animals, siRNA contains a seed region (2nd-7th nt) and a supplementary region (12th-17th nt). Based on the findings in shrimp and other animals, miRNAs are essential regulators of virus-host interactions, such as virus infection/latency, and host apoptosis, autophagy and phagocytosis. Except for the seed sequence (2nd-7th), the complementary bases (to the target mRNA sequence) of a miRNA 9th-18th non-seed sequence are essential for the miRNA targeting. So far, rapidly growing evidences have supported the existence of functional RNAi machinery in shrimp. In this review, we summarize the progress of RNAi in the antiviral immune response of shrimp. The potential applications of RNAi to control shrimp diseases were also summarized.

Keywords: Innate immunity; RNA interference (RNAi); Shrimp; Virus.