Altered IHH signaling contributes to reduced chondrocyte proliferation in the growth plate of MPS VII mice

Zhirui Jiang; Ainslie L K Derrick-Roberts; Sharon Byers

doi:10.1016/j.ymgmr.2020.100668

Altered IHH signaling contributes to reduced chondrocyte proliferation in the growth plate of MPS VII mice

Mol Genet Metab Rep. 2020 Oct 22:25:100668. doi: 10.1016/j.ymgmr.2020.100668. eCollection 2020 Dec.

Authors

Zhirui Jiang^{1

2}, Ainslie L K Derrick-Roberts^{2

3}, Sharon Byers^{1

2

3}

Affiliations

¹ Genetics and Evolution, The University of Adelaide, Adelaide, SA, Australia.
² Genetics and Molecular Pathology, SA Pathology, Adelaide, SA, Australia.
³ Paediatrics, The University of Adelaide, Adelaide, SA, Australia.

Abstract

Bone elongation is driven by chondrocyte proliferation and hypertrophy in the growth plate. Both processes are modulated by multiple signaling pathways including the Indian Hedgehog (IHH) signaling pathway. Mucopolysaccharidoses (MPS) are a group of lysosomal storage disorders characterized by accumulation of glycosaminoglycans (GAGs) in multiple tissues and organs, leading to a range of clinical symptoms including bone shortening through mechanisms that are not fully understood. Using MPS VII mice, we previously observed a reduction in the number of proliferating and hypertrophic chondrocytes and a reduced gene expression of Ihh in the tibial growth plate. We further demonstrate here that IHH secretion by MPS VII chondrocytes was reduced both in vitro and in vivo. While normal chondrocytes showed no response to exogenous IHH, proliferation of MPS VII chondrocytes was stimulated in response to exogenous IHH in vitro. This was accompanied by an elevated gene expression of patched receptor (Ptch1). The results from this study suggested that reduced proliferation in MPS VII growth plate may be partially due to dysfunction of the IHH signaling pathway.

Keywords: Bone shortening; Chondrocyte proliferation; Growth plate; Indian hedgehog (IHH); Mucopolysaccharidosis (MPS).