Amplified Fluorescent Aptasensor for Ochratoxin A Assay Based on Graphene Oxide and RecJf Exonuclease

Han Zhao; Dehui Xiong; Ying Yan; Changbei Ma

doi:10.3390/toxins12110670

Amplified Fluorescent Aptasensor for Ochratoxin A Assay Based on Graphene Oxide and RecJ_f Exonuclease

Toxins (Basel). 2020 Oct 23;12(11):670. doi: 10.3390/toxins12110670.

Authors

Han Zhao¹, Dehui Xiong¹, Ying Yan¹, Changbei Ma¹

Affiliation

¹ School of Life Sciences, Central South University, Changsha 410013, China.

Abstract

In this study, we developed an aptamer-based fluorescent sensing platform for the detection of ochratoxin A (OTA) based on RecJ_f exonuclease-assisted signal amplification and interaction between graphene oxide (GO) and the OTA aptamer (OTA-apt). After optimizing the experimental conditions, the present aptamer-based sensing system can exhibit excellent fluorescent response in the OTA assay, with a limit of detection of 0.07 ng/mL. In addition to signal amplification, this strategy is also highly specific for other interfering toxins. Furthermore, this aptasensor can be reliably used for assessing red wine samples spiked with different OTA concentrations (2.4, 6 and 20 ng/mL). The proposed assay plays an important role in the field of food safety and can be transformed for detecting other toxins by replacing the sequence that recognizes the aptamer.

Keywords: RecJf exonuclease; fluorescence; graphene oxide; ochratoxin A; signal amplification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aptamers, Nucleotide / chemistry*
Biological Assay
Exonucleases / chemistry*
Fluorescence
Food Contamination / analysis
Graphite / chemistry*
Ochratoxins / analysis*
Ochratoxins / chemistry
Wine / analysis

Substances

Aptamers, Nucleotide
Ochratoxins
graphene oxide
ochratoxin A
Graphite
Exonucleases