Capillary-driven blood separation and in-situ electrochemical detection based on 3D conductive gradient hollow fiber membrane

Huimin Wu; Chenfei Shi; Qin Zhu; Yang Li; Zhikang Xu; Chenjie Wei; Dajing Chen; Xiaojun Huang

doi:10.1016/j.bios.2020.112722

Capillary-driven blood separation and in-situ electrochemical detection based on 3D conductive gradient hollow fiber membrane

Biosens Bioelectron. 2021 Jan 1:171:112722. doi: 10.1016/j.bios.2020.112722. Epub 2020 Oct 14.

Authors

Huimin Wu¹, Chenfei Shi², Qin Zhu³, Yang Li¹, Zhikang Xu¹, Chenjie Wei⁴, Dajing Chen⁵, Xiaojun Huang⁶

Affiliations

¹ Key Laboratory of Macromolecular Synthesis and Functionalization, ERC of Membrane and Water Treatment (MOE), Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China.
² Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, China.
³ School of Medicine, Hangzhou Normal University, Hangzhou, 311121, China.
⁴ Key Laboratory of Macromolecular Synthesis and Functionalization, ERC of Membrane and Water Treatment (MOE), Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China. Electronic address: phdwei@zju.edu.cn.
⁵ School of Medicine, Hangzhou Normal University, Hangzhou, 311121, China. Electronic address: djchen@hznu.edu.cn.
⁶ Key Laboratory of Macromolecular Synthesis and Functionalization, ERC of Membrane and Water Treatment (MOE), Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China. Electronic address: hxjzxh@zju.edu.cn.

PMID: 33113384
DOI: 10.1016/j.bios.2020.112722

Abstract

It is essential to develop portable, versatile, and reliable diagnostic devices in point-of-care testing (POCT). The detection of biomarkers requires selective separation and large specific surface for high sensitivity and accuracy at trace levels in whole blood samples using POCT devices. Herein, a kind of 3D electrochemical biosensors were designed via in-situ synthesizing polyaniline (PANI) and platinum nanoparticles (Pt NPs) on polysulfone hollow fiber membrane (HFM) scaffolds with gradient porous structure. The gradient porous HFMs scaffolds provide uniform capillary flow, self-driven blood separation and sufficient enzyme immobilization sites. Simultaneously, the in-situ deposited materials fulfill interconnected conductive networks, thus ensuring accurate and rapid detection of the sensors without hindering capillary progress. These sensors display ultralow sampling (~3 μL), fast fluid flow (>1 μL/ms), wide linear range (glucose: 0-24 mM, R²=0.992; cholesterol: 0-9 mM, R²=0.999), high sensitivity and accuracy especially under different hematocrits in POCT applications towards glucose and cholesterol. The innovative integration of POCT biosensors with interconnected conductive nanoparticles, selective blood separation and gradient porous structures can find wide application in resource-limited regions, large population screening, and public health emergencies.

Keywords: Capillary; Metabolite; Point-of-care testing; Polyaniline; Polysulfone hollow fiber membrane.

MeSH terms

Biosensing Techniques*
Electric Conductivity
Glucose
Metal Nanoparticles*
Platinum

Substances

Platinum
Glucose