Subtype-Directed Differentiation of Human iPSCs into Atrial and Ventricular Cardiomyocytes

Mandy Kleinsorge; Lukas Cyganek

doi:10.1016/j.xpro.2020.100026

Subtype-Directed Differentiation of Human iPSCs into Atrial and Ventricular Cardiomyocytes

STAR Protoc. 2020 Jun 3;1(1):100026. doi: 10.1016/j.xpro.2020.100026. eCollection 2020 Jun 19.

Authors

Mandy Kleinsorge^{1

2}, Lukas Cyganek^{1

2}

Affiliations

¹ Clinic for Cardiology and Pneumology, University Medical Center Göttingen, 37075 Göttingen, Germany.
² DZHK (German Center for Cardiovascular Research), partner site Göttingen, Göttingen, Germany.

Abstract

The generation of homogeneous populations of subtype-specific cardiomyocytes derived from human induced pluripotent stem cells (hiPSCs) is crucial in cardiovascular disease modeling as well as in drug discovery and cardiotoxicity screenings. This protocol describes a simple, robust, and efficient monolayer-based differentiation of hiPSCs into defined atrial and ventricular cardiomyocytes. For complete details on the use and execution of this protocol, please refer to Cyganek et al., 2018.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Culture Techniques / methods*
Cell Differentiation / physiology*
Cells, Cultured
Heart Atria / cytology
Heart Ventricles / cytology
Humans
Induced Pluripotent Stem Cells* / cytology
Induced Pluripotent Stem Cells* / physiology
Myocytes, Cardiac* / cytology
Myocytes, Cardiac* / physiology