CRISPR-Cas9-Based Genome Editing and Cytidine Base Editing in Acinetobacter baumannii

Yu Wang; Zhipeng Wang; Quanjiang Ji

doi:10.1016/j.xpro.2020.100025

CRISPR-Cas9-Based Genome Editing and Cytidine Base Editing in Acinetobacter baumannii

STAR Protoc. 2020 Jun 3;1(1):100025. doi: 10.1016/j.xpro.2020.100025. eCollection 2020 Jun 19.

Authors

Yu Wang^{1

2}, Zhipeng Wang¹, Quanjiang Ji¹

Affiliations

¹ School of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, China.
² College of Life Science and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China.

Abstract

Infections caused by drug-resistant Acinetobacter baumannii have posed a serious threat to global public health. However, genetic manipulation methods, the primary way to study pathogenesis and drug-resistance mechanisms, remain time consuming and inefficient. Here, we provide a detailed protocol for genetic manipulation, including gene deletion, insertion, and point mutation in A. baumannii using the platform. For complete details on the use and execution of this protocol, please refer to Wang et al. (2019).

MeSH terms

Acinetobacter baumannii / genetics*
CRISPR-Cas Systems / genetics*
Cytidine / genetics*
Electroporation
Gene Editing / methods*
Point Mutation / genetics

Substances

Cytidine