Chromatin-Bound Proteome Profiling by Genome Capture

STAR Protoc. 2020 Jun 3;1(1):100014. doi: 10.1016/j.xpro.2020.100014. eCollection 2020 Jun 19.

Abstract

De novo identification of chromatin interactors can reveal unexpected pathways relevant to physiology and human disease. Inspired by the DNA mediated chromatin pull-down (Dm-ChP) technology (also known as iPOND [isolation of proteins on nascent DNA]) for the proteomic characterization of nascent DNA, we have recently reported a new experimental protocol that allows for the identification of proteins on total DNA (iPOTD) for bulk chromatome profiling and de novo identification of chromatin-bound proteins. Here, we detail a step-by-step protocol to survey the cellular chromatin-bound proteome in a simple, robust, and unbiased manner. For complete details on the use and execution of this protocol, please refer to Aranda et al. (2019).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Chromatin* / chemistry
  • Chromatin* / metabolism
  • Genetic Techniques*
  • Genome / genetics
  • Humans
  • Proteome* / analysis
  • Proteome* / chemistry
  • Proteome* / genetics
  • Proteome* / metabolism
  • Proteomics / methods*

Substances

  • Chromatin
  • Proteome