Establishment and Long-Term Culture of Organoids Derived from Human Biliary Tract Carcinoma

Yoshimasa Saito; Toshihide Muramatsu; Hidetsugu Saito

doi:10.1016/j.xpro.2019.100009

Establishment and Long-Term Culture of Organoids Derived from Human Biliary Tract Carcinoma

STAR Protoc. 2020 Jun 3;1(1):100009. doi: 10.1016/j.xpro.2019.100009. eCollection 2020 Jun 19.

Authors

Yoshimasa Saito¹, Toshihide Muramatsu¹, Hidetsugu Saito¹

Affiliation

¹ Division of Pharmacotherapeutics, Keio University Faculty of Pharmacy, 1-5-30 Shibakoen, Minato-ku, Tokyo 105-8512, Japan.

Abstract

This protocol is a procedure for establishment and culture of cancer and non-cancer organoids using tissues from biliary tract carcinoma (BTC) patients. These BTC organoids can be used for various biological analyses and drug screening. One challenge in establishing and culturing BTC organoids is non-cancer cells contaminating surgically resected tumor tissues form organoids concurrently with cancer organoids. Careful validation that the established organoids are cancer-derived is important. For complete details on the use and generation of this protocol, please refer to Saito et al. (2019) in the journal Cell Reports.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biliary Tract / cytology*
Biliary Tract Neoplasms*
Carcinoma*
Cell Culture Techniques / methods*
Humans
Organoids* / cytology
Organoids* / metabolism
Tissue Culture Techniques / methods*