Generation of Human iMelanocytes from Induced Pluripotent Stem Cells through a Suspension Culture System

Li-Ping Liu; Ning-Ning Guo; Yu-Mei Li; Yun-Wen Zheng

doi:10.1016/j.xpro.2019.100004

Generation of Human iMelanocytes from Induced Pluripotent Stem Cells through a Suspension Culture System

STAR Protoc. 2020 Jun 3;1(1):100004. doi: 10.1016/j.xpro.2019.100004. eCollection 2020 Jun 19.

Authors

Li-Ping Liu^{1

2

3}, Ning-Ning Guo^{1

2}, Yu-Mei Li^{1

2}, Yun-Wen Zheng^{1

3

4

5}

Affiliations

¹ Institute of Regenerative Medicine, Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, Jiangsu 212001, China.
² Department of Dermatology, Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, Jiangsu 212001, China.
³ University of Tsukuba Faculty of Medicine, Ibaraki 305-8575, Japan.
⁴ Yokohama City University School of Medicine, Yokohama 236-0004, Japan.
⁵ Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan.

Abstract

Melanocytes, derived from neural crest cells, are involved in melanin production. This protocol describes a method to generate induced melanocytes (iMelanocytes) from human induced pluripotent stem cells (iPSCs) using a suspension culture system, which considerably improves the differentiation efficiency. The most critical parts of this protocol are the selection of a reliable iPSC line with strong potential to differentiate into melanocytes and their stemness maintenance. For complete information on the use and generation of this protocol, please refer to our Cell Reports article, Liu el al. (2019).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Culture Techniques / methods*
Cell Differentiation / physiology
Cells, Cultured
Humans
Induced Pluripotent Stem Cells / cytology*
Melanocytes / cytology*
Neural Crest / cytology