Association of specific microorganisms with endodontic signs and symptoms. A comparative study

Kishore Kumar Singh; Pankaj Kumar; Pragyan Das; Manjula Marandi; Swagat Panda; Amit Mahajan; Dinesh Kumar

doi:10.4103/jfmpc.jfmpc_523_20

Association of specific microorganisms with endodontic signs and symptoms. A comparative study

J Family Med Prim Care. 2020 Aug 25;9(8):3965-3969. doi: 10.4103/jfmpc.jfmpc_523_20. eCollection 2020 Aug.

Authors

Kishore Kumar Singh¹, Pankaj Kumar², Pragyan Das³, Manjula Marandi⁴, Swagat Panda⁵, Amit Mahajan⁶, Dinesh Kumar⁷

Affiliations

¹ Department of Psychiatry, Nalanda Medical College, Patna, Bihar, India.
² Department of Conservative Dentistry and Endodontics, Awadh Dental College and Hospital, Jamshedpur, Jharkhand, India.
³ Department of Oral Medicine and Radiology, Awadh Dental College and Hospital, Jamshedpur, Jharkhand, India.
⁴ Department of Oral Pathology, Microbiology and Forensic Odontology, Rajendra Institute of Medical Sciences, Dental Institute, Ranchi, Jharkhand, India.
⁵ Department of Conservative Dentistry and Endodontics, Hi-tech Dental College & Hospital, Bhubaneswar, Odisha, India.
⁶ Department of Conservative Dentistry and Endodontics, Rama Dental College and Hospital, Kanpur, UP, India.
⁷ BRS Dental College and Hospital, Sultanpur, Panchkula, Haryana, India.

Abstract

Aim: The study aimed to evaluate the association of root canal microorganisms red complex and E. facealis with endodontic clinical signs and symptoms using polymerase chain reaction.

Materials and methods: Bacterial samples were obtained using sterile paper points from the teeth of 100 subjects divided into two groups; Group I: 50 individuals with primary tooth infections and Group II: 50 individuals with failed endodontic treatment having the secondary infection. DNA extracted from samples was analyzed for endodontic pathogens by using species-specific primers.

Results: The pain was noticed in 66%, (33 of 50 subjects) in primary infection and 60% (30 of 50) in a secondary infection. A statistically significant association between pain and E. faecalis bacteria observed both in primary infection and secondary infection (P < 0.05). Tenderness on percussion was associated with 40% cases in Group I and 70% cases in Group II. The red complex accounted for 94% of cases associated with tenderness on percussion in primary infection while 86% of cases associated with secondary infection with a statistically significant association (P < 0.05).

Conclusion: Prevalence of red complex bacteria and E. faecalis suggested the association of studied bacteria with symptomatic infected pulp and periradicular diseases.

Keywords: Bacteria; pulp; red complex.