Abstract
Viruses engineered to express fluorescent proteins can be used with live-cell imaging techniques to monitor the progression of infection in real time. Here we describe a set of methods to track infection spreading from one cell population to another as well as to visualize transfer of virions between cells. This approach is extended to multiplexing with physiological readouts of cell death, which can be correlated with single-cell resolution to viral infection.
Keywords:
Apoptosis; Cell death; Confocal; Fluorescence; Infection; Live-cell imaging; Myxoma virus; Viruses.
MeSH terms
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A549 Cells
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Apoptosis / genetics
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Cell Tracking / methods*
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Gene Expression
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Genes, Reporter
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Genetic Engineering / methods*
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Humans
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Jurkat Cells
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Luminescent Proteins / genetics
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Luminescent Proteins / metabolism
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Myxoma virus / genetics*
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Myxoma virus / growth & development
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Myxoma virus / metabolism
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Optical Imaging / methods*
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Optical Imaging / statistics & numerical data
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Red Fluorescent Protein
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Viral Proteins / genetics*
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Viral Proteins / metabolism
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Virion / genetics*
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Virion / growth & development
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Virion / metabolism
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Virus Replication / genetics
Substances
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Luminescent Proteins
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Viral Proteins
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Green Fluorescent Proteins