This study mainly discussed the effect of fermented Huyou juice (FHJ) on modulating the intestinal microbiota of human, and anti-obesity mechanisms. Through the way of metagenomics, the effect of FHJ on gut flora has been summarized with a mice model of obesity induced by human flora-associated (HFA) high-fat diet. The results showed that the FHJ ameliorated the gut dysbiosis caused by obesity. When receiving FHJ treatment, a dramatic decrease in Firmicutes/Bacteroidetes occurred. What's more, having experienced 8 weeks of FHJ intervention, KEGG pathways of two-component system, ATP-binding cassette (ABC) transporters, and biosynthesis of amino acids made the most differentially expressed genes more abundant, the unigene numbers are 16781,480, and 1,221, respectively. Our results may be of great significance to the use of FHJ which serves as a functional fermented beverage product with the underlying effect of treating the obesity induced by high-fat diet. The FHJ helps to improve the host health by regulating the intestinal flora and affecting some metabolic pathways. PRACTICAL APPLICATIONS: The fermentation of Huyou juice is one of the important ways to develop and utilize fruit resources. It is a common way of fruit and vegetable juice fermentation with mixed strains. After fermentation, the juice produces a large number of bioactive peptides, and sugar, toxic substances, and antinutritional material will be reduced, the nutritional value of the fruits and vegetables were improved. At the same time, the fermented juice industry could develop various functional health products, which is conducive to the transformation, upgrading, and sustainable development of Changshan Huyou.
Keywords: fermented Huyou juice; intestinal microbiota; metagenomics; modulatory effect; obesity.
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