Species diversity and distribution of Anopheles mosquitoes in Bure district, Northwestern Ethiopia

Tilahun Adugna; Emana Getu; Delenasaw Yewhalaw

doi:10.1016/j.heliyon.2020.e05063

Species diversity and distribution of Anopheles mosquitoes in Bure district, Northwestern Ethiopia

Heliyon. 2020 Oct 16;6(10):e05063. doi: 10.1016/j.heliyon.2020.e05063. eCollection 2020 Oct.

Authors

Tilahun Adugna¹, Emana Getu², Delenasaw Yewhalaw³

Affiliations

¹ Debre Tabor University, P.O. Box: 272, Debre Tabor, Ethiopia.
² Addis Ababa University, P.O. Box: 2003, Addis Ababa, Ethiopia.
³ Jima University, P.O. Box: 378, Jima, Ethiopia.

Abstract

Malaria is one the leading health problem of the Ethiopia. Previously, areas above 2,000 m elevation were considered as malaria free areas. However, the major malaria epidemics were seen in areas at an altitude up to 3,000 m above sea level. These epidemics were due to climate and land-use changes (ecological changes) and still malaria is a growing health problem in highland parts of Ethiopia. This study aimed to investigate the species diversity, abundance and distribution of Anopheles mosquitoes in highland fringe of Bure district, Northwestern Ethiopia. It was done in the three different agroecological villages, Bukta (Irrigated), Workimdr (non-irrigated with few dry season breeding habitats) and Shnebekuma (non-irrigated with many dry season breeding habitats). Anopheles mosquitoes were collected by the Centers for Disease Control and Prevention Light Traps Catches, Pyrethrum Spray Catches, and Artificial Pit Shelters (APSs) from twenty-seven houses, thirty houses, and six APSs, respectively. Anopheles mosquitoes were identified morphologically to species using standard keys. Furthermore, molecular identification of Anopheles gambiae s.l was carried out using species-specific Polymerase Chain Reaction. Independent T-Test and One-way- ANOVA were employed to compare the mean mosquito's density between villages and species, indoor and outdoor host seeking mosquitoes. Descriptive statistic was used to calculate the proportion of each Anopheles species. Nine Anopheles mosquito species were identified in the study area which includes: Anopheles demeilloni, An. arabiensis, An. funestus group, An. coustani, An. squamosus, An. cinereus, An. pharoensis, An. rupicolus, and An. natalensis. Of the 4,703 Anopheles mosquitoes collected, An. demeilloni was the most prominent (50.7%, n = 2383) whereas An. rupicolus (0.03%, n = 3), and An. natalensis (0.02%, n = 1) were the least abundant. Higher mean density of Anopheles mosquitoes was collected from the non-irrigated village (2.395 ± 0.100) than irrigated (1.351 ± 0.109) (p = 0.001). In conclusion, three of the most important malaria vectors (An. arabiensis, An. funestus group and An. pharoensis) of Ethiopia were recorded in the study sites, especially the first two was found thought-out the year. Most of the Anopheles mosquitoes were collected from non-irrigated villages. Thus, breeding habitat management must be practiced throughout the year together with long-lasting insecticide-treated nets and insecticide residual sprays.

Keywords: Anopheles arabiensis; Anopheles funestus group; Bure district; Ecology; Highland part; Malaria; Molecular biology; Non-irrigated village; Zoology.