Whole genome sequence of Serratia marcescens 39_H1, a potential hydrolytic and acidogenic strain

Linda U Obi; Memory Tekere; Ashira Roopnarain; Tomasz Sanko; Tawanda E Maguvu; Cornelius C Bezuidenhout; Rasheed A Adeleke

doi:10.1016/j.btre.2020.e00542

Whole genome sequence of Serratia marcescens 39_H1, a potential hydrolytic and acidogenic strain

Biotechnol Rep (Amst). 2020 Oct 9:28:e00542. doi: 10.1016/j.btre.2020.e00542. eCollection 2020 Dec.

Authors

Linda U Obi^{1

2}, Memory Tekere¹, Ashira Roopnarain², Tomasz Sanko³, Tawanda E Maguvu³, Cornelius C Bezuidenhout³, Rasheed A Adeleke^{2

3}

Affiliations

¹ Department of Environmental Sciences, University of South Africa, Johannesburg, South Africa.
² Microbiology and Environmental Biotechnology Research Group, Institute for Soil, Climate and Water, Agricultural Research Council, Arcadia, 0083, Pretoria, South Africa.
³ Unit for Environment Sciences and Management, North-West University (Potchefstroom Campus), Potchefstroom, South Africa.

Abstract

Here, we report a high quality annotated draft genome of Serratia marcescens 39_H1, a Gram-negative facultative anaerobe that was isolated from an anaerobic digester. The strain exhibited hydrolytic/acidogenic properties by significantly improving methane production when used as a single isolate inoculum during anaerobic digestion of water hyacinth and cow dung. The total genome size of the isolate was 5,106,712 bp which corresponds to an N50 of 267,528 and G + C content of 59.7 %. Genome annotation with the NCBI Prokaryotic Genome Annotation Pipeline (PGAP) predicted a total of 4,908 genes of which 4,755 were protein coding genes; there were no plasmids detected. A number of genes associated with hydrolytic/acidogenic activities as well as other metabolic activities were identified and discussed.

Keywords: Biogas; Hydrolysis; Plant-growth-promotion; Serratia marcescens; Whole genome sequence.