Superagonistic CD28 stimulation induces IFN-γ release from mouse T helper 1 cells in vitro and in vivo

Stephanie Haack; Sarah Baiker; Jan Schlegel; Markus Sauer; Tim Sparwasser; Daniela Langenhorst; Niklas Beyersdorf

doi:10.1002/eji.202048803

Superagonistic CD28 stimulation induces IFN-γ release from mouse T helper 1 cells in vitro and in vivo

Eur J Immunol. 2021 Mar;51(3):738-741. doi: 10.1002/eji.202048803. Epub 2020 Dec 8.

Authors

Stephanie Haack¹, Sarah Baiker¹, Jan Schlegel², Markus Sauer², Tim Sparwasser³, Daniela Langenhorst¹, Niklas Beyersdorf¹

Affiliations

¹ Institute for Virology and Immunobiology, University of Würzburg, Würzburg, Germany.
² University of Würzburg, Biotechnology and Biophysics, Würzburg, Germany.
³ Johannes Gutenberg University Mainz, Institute for Medical Microbiology and Hygiene, Mainz, Germany.

PMID: 33098656
DOI: 10.1002/eji.202048803

Abstract

Like human Th1 cells, mouse Th1 cells also secrete IFN-γ upon stimulation with a superagonistic anti-CD28 monoclonal antibody (CD28-SA). Crosslinking of the CD28-SA via FcR and CD40-CD40L interactions greatly increased IFN-γ release. Our data stress the utility of the mouse as a model organism for immune responses in humans.

Keywords: CD28; Superagonistic antibody; Th1 cells; cytokine release; interferon γ.

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Monoclonal / immunology
CD28 Antigens / immunology*
CD40 Antigens / immunology
CD40 Ligand / immunology
Humans
Interferon-gamma / immunology*
Lymphocyte Activation / immunology
Mice
Signal Transduction / immunology
Th1 Cells / immunology*

Substances

Antibodies, Monoclonal
CD28 Antigens
CD40 Antigens
CD40 Ligand
Interferon-gamma