14C is known as one of the radionuclides that have potential to be released into the biosphere from radioactive waste repositories and taken up by organisms. In this study, we used a novel approach to investigate the proportion of soil organic carbon (SOC) in invertebrates and microbial biomass. The study was conducted on a peatland site after the end of peat extraction. There was a large difference in the isotopic abundance of 14C between the 8000-year-old peat and air. We used a two-pool isotope mixing model to reveal the fraction of soil-derived C in the organisms and in dissolved organic carbon in soil water. The contribution of soil-derived C was found to be highest in microbial biomass (61%) and earthworms (22%). Some contribution of soil-derived C was detected in fungus gnats (2%), but not in other insects or in spiders. These findings are important for developing evidence-based radioecological models based on correct understanding of the relative contributions of atmospheric C vs. SOC in organisms.
Keywords: C-14; Invertebrates; Microbial biomass; Radiocarbon; Radioecology; Two-pool isotope mixing model.
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