Cyclodextrin glycosyltransferase-treated germinated brown rice flour improves the cytotoxic capacity of HepG2 cell and has a positive effect on type-2 diabetic mice

Nguyen Duong Thanh Binh; Nguyen Thi Le Ngoc; Ibitoye Joshua Oladapo; Co Hong Son; Do Thi Thao; Dai Thi Xuan Trang; Tong Thi Anh Ngoc; Nguyen Cong Ha

doi:10.1111/jfbc.13533

Cyclodextrin glycosyltransferase-treated germinated brown rice flour improves the cytotoxic capacity of HepG2 cell and has a positive effect on type-2 diabetic mice

J Food Biochem. 2020 Dec;44(12):e13533. doi: 10.1111/jfbc.13533. Epub 2020 Oct 22.

Authors

Nguyen Duong Thanh Binh¹, Nguyen Thi Le Ngoc¹, Ibitoye Joshua Oladapo¹, Co Hong Son², Do Thi Thao³, Dai Thi Xuan Trang⁴, Tong Thi Anh Ngoc¹, Nguyen Cong Ha¹

Affiliations

¹ Food Technology Department, College of Agriculture, Campus II, Can Tho University, Can Tho City, Vietnam.
² National Agro - Forestry - Fishery Quality Assurance Department, Branch 6 (NAFIQUAD), Can Tho City, Vietnam.
³ Institute of Biotechnology, Vietnam Academy of Science and Technology (VAST), Hanoi, Vietnam.
⁴ Department of Biology, College of Natural Science, Campus II, Can Tho University, Can Tho City, Vietnam.

PMID: 33094483
DOI: 10.1111/jfbc.13533

Abstract

Germinated brown rice (GBR) consists of bioactive compounds (BCs) that are very useful for diabetes treatment. Modified GBR-based flour (MGBRF) was produced by modifying the starch in GBR with 0, 299.19, 598.38, and 897.57 U/ml of cyclodextrin glycosyltransferase (CGTase) for 1 hr and then spray-dried to examine its antidiabetic and cytotoxic effects. The results showed that the slowly digestible starch and resistant starch by modifying the starch in GBR with 598.38 U/ml of CGTase were 55.8% and 5.92% corresponding to the increase of γ-amino butyric acid (GABA) and ferulic acid (FA) with 4.31 ± 0.68 mg/ml and 3.10 ± 0.02 mg/ml, respectively. The extract from MGBRF showed strong cytotoxic capacity against HepG2. Furthermore, the in vivo study revealed the stability of the glycemic index (GI) by consuming MGBRF with significant impacts on diabetes. These results suggest that MGBRF through the action of CGTase plays a major role in antidiabetes and HepG2 cell product value addition. PRACTICAL APPLICATIONS: GBR consists of BCs that are useful for diabetes and cancer treatment. However, when using this or GBR-based products, it is difficult to evaluate the effect of functional properties, especially for diabetes and/or cancer diseases due to high starch content. Therefore, the modification of starch to limit digestible starch, increase SDS and RS as well as to enhance the effect of BCs on diabetes and cytotoxic activity on cancer cell should be studied before producing various based products from GBR. The results in this study indicated that CGTase increased BCs without any glycosides BCs in the extract. The MGBRF changed to higher RS and SDS while increasing the BCs. The extract of MGBRF showed strong cytotoxic activity against HepG2 cell and a positive effect on type 2-diabetic mice. Hence, this study produces new information for effective use of GBR-based food as a functional food.

Keywords: bioactive compounds; cyclodextrin glycosyltransferase; germinated brown rice; resistant starch; slowly digestible starch.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Diabetes Mellitus, Experimental* / drug therapy
Diabetes Mellitus, Type 2*
Flour
Germination
Glucosyltransferases
Hep G2 Cells
Mice
Oryza*

Substances

Glucosyltransferases
cyclomaltodextrin glucanotransferase