Effect of APTES modified TiO2 on antioxidant enzymes activity secreted by Escherichia coli and Staphylococcus epidermidis

Biochem Biophys Res Commun. 2021 Jan 1:534:1064-1068. doi: 10.1016/j.bbrc.2020.10.034. Epub 2020 Oct 20.

Abstract

In this work, the impact of APTES-modified TiO2 photocatalysts on antioxidant enzymes (catalase and superoxide dismutase) activity secreted by bacteria was presented. Microbial tests has been examined using Escherichia coli (ATCC 29425) and Staphylococcus epidermidis (ATCC 49461) as model organisms. It was found that APTES-TiO2 affected the activity of antioxidant enzymes. Additionally, obtained APTES-TiO2 photocatalysts were capable of total E. coli and S. epidermidis inactivation under artificial solar light irradiation. The sample modified with the concentration of APTES equals 300 mM (TiO2-4h-120°C-300mM) showed the strongest photocatalytic activity toward both bacteria species. The two-stage photocatalytic mechanism of bacteria response to photocatalysts was proposed.

Keywords: 3-Aminopropyltriethoxysilane (APTES); Catalase (CAT); Enzymatic activity; Photocatalysis; Superoxide dismutase (SOD).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Catalase / metabolism*
  • Catalysis / radiation effects
  • Disinfection
  • Enzyme Activation / radiation effects
  • Escherichia coli / cytology
  • Escherichia coli / enzymology*
  • Escherichia coli / radiation effects
  • Light
  • Microbial Viability / radiation effects
  • Oxidative Stress / radiation effects
  • Photochemical Processes / radiation effects
  • Propylamines / chemistry*
  • Silanes / chemistry*
  • Staphylococcus epidermidis / cytology
  • Staphylococcus epidermidis / enzymology*
  • Staphylococcus epidermidis / radiation effects
  • Superoxide Dismutase / metabolism*
  • Titanium / chemistry*

Substances

  • Propylamines
  • Silanes
  • titanium dioxide
  • Titanium
  • Catalase
  • Superoxide Dismutase
  • amino-propyl-triethoxysilane