Recent Progress and Development of G-Quadruplex-Based Luminescent Assays for Ochratoxin A Detection

Sang-Cuo Nao; Ke-Jia Wu; Wanhe Wang; Chung-Hang Leung; Dik-Lung Ma

doi:10.3389/fchem.2020.00767

Recent Progress and Development of G-Quadruplex-Based Luminescent Assays for Ochratoxin A Detection

Front Chem. 2020 Sep 1:8:767. doi: 10.3389/fchem.2020.00767. eCollection 2020.

Authors

Sang-Cuo Nao¹, Ke-Jia Wu¹, Wanhe Wang², Chung-Hang Leung¹, Dik-Lung Ma²

Affiliations

¹ State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, China.
² Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, China.

Abstract

Ochratoxin A (OTA) is a mycotoxin that is widespread throughout the world. It contaminates foods such as vegetables, fruits, and rice. It harms human health and has potential carcinogenic effects. The G-quadruplex (G4) is a tetraplexed DNA structure generated from guanine-rich DNA that has found emerging use in aptamer-based sensing systems. This review outlines the status of OTA contamination and conventional detection methods for OTA. Various G4-based methods to detect OTA developed in recent years are summarized along with their advantages and disadvantages compared to existing approaches.

Keywords: G-quadruplex; aptamer; food safety; nucleic acid detection; ochratoxin A.

Publication types

Review