Long non-coding RNA LINC01234 regulates proliferation, migration and invasion via HIF-2α pathways in clear cell renal cell carcinoma cells

Feilong Yang; Cheng Liu; Guojiang Zhao; Liyuan Ge; Yimeng Song; Zhigang Chen; Zhuo Liu; Kai Hong; Lulin Ma

doi:10.7717/peerj.10149

Long non-coding RNA LINC01234 regulates proliferation, migration and invasion via HIF-2α pathways in clear cell renal cell carcinoma cells

PeerJ. 2020 Oct 14:8:e10149. doi: 10.7717/peerj.10149. eCollection 2020.

Authors

Feilong Yang¹, Cheng Liu¹, Guojiang Zhao¹, Liyuan Ge¹, Yimeng Song¹, Zhigang Chen¹, Zhuo Liu¹, Kai Hong¹, Lulin Ma¹

Affiliation

¹ Department of Urology, Peking University Third Hospital, Beijing, China.

Abstract

Long non-coding RNAs (lncRNAs) have been proved to have an important role in different malignancies including clear cell renal cell carcinoma (ccRCC). However, their role in disease progression is still not clear. The objective of the study was to identify lncRNA-based prognostic biomarkers and further to investigate the role of one lncRNA LINC01234 in progression of ccRCC cells. We found that six adverse prognostic lncRNA biomarkers including LINC01234 were identified in ccRCC patients by bioinformatic analysis using The Cancer Genome Atlas database. LINC01234 knockdown impaired cell proliferation, migration and invasion in vitro as compared to negative control. Furthermore, the epithelial-mesenchymal transition was inhibited after LINC01234 knockdown. Additionally, LINC01234 knockdown impaired hypoxia-inducible factor-2a (HIF-2α) pathways, including a suppression of the expression of HIF-2α, vascular endothelial growth factor A, epidermal growth factor receptor, c-Myc, Cyclin D1 and MET. Together, these datas showed that LINC01234 was likely to regulate the progression of ccRCC by HIF-2α pathways, and LINC01234 was both a promising prognostic biomarker and a potential therapeutic target for ccRCC.

Keywords: Bioinformatics; Cell Invasion; Cell migration; Cell proliferation; Clear cell renal cell carcinoma; EMT; HIF-2α pathways; LINC01234; lncRNA.

Grants and funding

The study was funded by the National Natural Science Foundation of China (No. 81972381), the National Key Research and Development Program of China (grant nos. 2017YFC1002001 and SQ2018YFC100243), the Beijing Natural Science Foundation of China (7182177) and the Beijing Municipal Science & Technology Commission (grant no. Z151100003915105). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.