Anti-adipogenic effect of Lactobacillus fermentum MG4231 and MG4244 through AMPK pathway in 3T3-L1 preadipocytes

SukJin Kim; Soo-Im Choi; Miran Jang; Yulah Jeong; Chang-Ho Kang; Gun-Hee Kim

doi:10.1007/s10068-020-00819-2

Anti-adipogenic effect of Lactobacillus fermentum MG4231 and MG4244 through AMPK pathway in 3T3-L1 preadipocytes

Food Sci Biotechnol. 2020 Oct 6;29(11):1541-1551. doi: 10.1007/s10068-020-00819-2. eCollection 2020 Nov.

Authors

SukJin Kim¹, Soo-Im Choi¹, Miran Jang², Yulah Jeong³, Chang-Ho Kang³, Gun-Hee Kim^{1

4}

Affiliations

¹ Department of Bio-Health Convergence Major, Duksung Women's University, Seoul, 01369 Republic of Korea.
² Department of Food Science, Purdue University, West Lafayette, IN 47906 USA.
³ MEDIOGEN, Co., Ltd., Jecheon, 27159 Republic of Korea.
⁴ Department of Food and Nutrition, Duksung Women's University, Seoul, 01369 Republic of Korea.

Abstract

This study evaluated the anti-adipogenic effects and mechanisms underlying the action of Lactobacillus fermentum MG4231 and MG4244 strains on adipogenesis and lipid accumulation in 3T3-L1 preadipocytes. Treatment with cell-free extracts (CFEs) from the two strains reduced lipid accumulation and intracellular triglyceride production in 3T3-L1 adipocytes by more than 50%. The inhibitory effects of L. fermentum on lipid accumulation were mediated by the downregulation of FAS and aP2 resulting from the inhibition of PPARγ and C/EBPα gene expression. Moreover, AMPK and HSL phosphorylation was upregulated by CFE treatment. These results indicated that the anti-adipogenic and lipolysis activities of L. fermentum strains were caused by increased AMPK and HSL phosphorylation. Both strains displayed high leucine arylamidase and β-galactosidase enzymatic activity, with excellent adhesion to epithelial cells. Therefore, we identified L. fermentum as potential new probiotics for the prevention of obesity.

Keywords: Adipogenesis; L. fermentum; MG4231; MG4244; Probiotics.