Study of T-cell immunoglobulin and mucin domain-3 expression profile in peripheral blood and bone marrow of human acute lymphoblastic leukemia patients

Narges Zargar Balajam; Mahdi Shabani; Mahmoud Aghaei; Mansoureh Haghighi; Farzad Kompani

doi:10.4103/jrms.JRMS_759_19

Study of T-cell immunoglobulin and mucin domain-3 expression profile in peripheral blood and bone marrow of human acute lymphoblastic leukemia patients

J Res Med Sci. 2020 Jul 27:25:69. doi: 10.4103/jrms.JRMS_759_19. eCollection 2020.

Authors

Narges Zargar Balajam¹, Mahdi Shabani², Mahmoud Aghaei¹, Mansoureh Haghighi¹, Farzad Kompani³

Affiliations

¹ Department of Clinical Biochemistry, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran.
² Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
³ Department of Hematology and Oncology, Children's Hospital Medical Center, Tehran University of Medical Sciences, Tehran, Iran.

Abstract

Background: Acute lymphoblastic leukemia (ALL) is a malignancy with aggressive tumors of immature lymphocytes. T-cell immunoglobulin and mucin-domain 3 (TIM-3) is a Type I transmembrane glycoprotein which is involved in cell proliferation. The objective of this research is to determine the TIM-3 expression in peripheral blood (PB) and bone marrow (BM) of 80 samples of normal and ALL patients.

Materials and methods: The amount of mRNA and protein of TIM-3 measured in the BM and PB the mononuclear layer of samples by real-time polymerase chain reaction and Western blotting.

Results: Our findings indicated that relative mRNA expression of TIM-3 in PB and BM of the mononuclear layer of ALL patients was 1.7 and 5 times higher than normals, respectively. We also reported that the protein level of TIM-3 in mononuclear cells of ALL patients was 3.2-fold in BM and two-fold in PB more than normals.

Conclusion: In conclusion, this study shows that TIM-3 increases in ALL patients, thus the expression of TIM-3 in tumor cells may be considered as a potential predictive factor in ALL patients, which needs to be explored in future.

Keywords: Gene expression profiling; T-cell immunoglobulin and mucin-domain 3; precursor cell lymphoblastic leukemia-lymphoma; real-time polymerase chain reaction.