Quantification of Light Scattering Detection Efficiency and Background in Flow Cytometry

Leonie de Rond; Frank A W Coumans; Joshua A Welsh; Rienk Nieuwland; Ton G van Leeuwen; Edwin van der Pol

doi:10.1002/cyto.a.24243

Quantification of Light Scattering Detection Efficiency and Background in Flow Cytometry

Cytometry A. 2021 Jul;99(7):671-679. doi: 10.1002/cyto.a.24243. Epub 2020 Nov 7.

Authors

Leonie de Rond^{1

2

3}, Frank A W Coumans^{1

2

3}, Joshua A Welsh⁴, Rienk Nieuwland^{2

3}, Ton G van Leeuwen^{1

3}, Edwin van der Pol^{1

2

3}

Affiliations

¹ Biomedical Engineering and Physics, Amsterdam UMC, University of Amsterdam, Amsterdam, the Netherlands.
² Laboratory Experimental Clinical Chemistry, Amsterdam UMC, University of Amsterdam, Amsterdam, the Netherlands.
³ Vesicle Observation Center, Amsterdam UMC, University of Amsterdam, Amsterdam, the Netherlands.
⁴ Center for Cancer Research, National Cancer Institute, National Institute of Health, Bethesda, Maryland, USA.

No abstract available

Keywords: background light; detection limit; flow cytometry; light scattering; optical efficiency; photoelectron scale; resolution limit; sensitivity; standardization.

Quantification of Light Scattering Detection Efficiency and Background in Flow Cytometry

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