The diterpene spiroviolene, its diterpene synthase from Streptomyces violens and the experimentally determined terpene cyclisation mechanism were reported in 2017. Recently, the structure of spiroviolene was revised based on a total synthesis, with consequences for the cyclisation mechanism. Herein, a reinvestigation of the terpene cyclisation to spiroviolene and the mass spectrometric fragmentation mechanism investigated by 13 C-labelling experiments are presented.
Keywords: biosynthesis; enzyme mechanisms; isotopes; mass spectrometry; terpenes.
© 2020 The Authors. ChemBiochem published by Wiley-VCH GmbH.