An ICT-based fluorescent probe guided by theoretical calculation for selectively mapping endogenous GSH in living cells

Jiao Chen; Yiwei Li; Xukai Feng; Mengyao She; Wenxin Yao; Xin Leng; Ping Liu; Shengyong Zhang; Jianli Li

doi:10.1016/j.saa.2020.119041

An ICT-based fluorescent probe guided by theoretical calculation for selectively mapping endogenous GSH in living cells

Spectrochim Acta A Mol Biomol Spectrosc. 2021 Feb 5:246:119041. doi: 10.1016/j.saa.2020.119041. Epub 2020 Oct 6.

Authors

Jiao Chen¹, Yiwei Li¹, Xukai Feng¹, Mengyao She², Wenxin Yao¹, Xin Leng², Ping Liu¹, Shengyong Zhang¹, Jianli Li³

Affiliations

¹ Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi province 710127, PR China.
² Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi province 710127, PR China; Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Biomedicine Key Laboratory of Shaanxi Province, Lab of Tissue Engineering, the College of Life Sciences, Faculty of Life Science & Medicine, Northwest University, Xi'an, Shaanxi Province 710069, PR China.
³ Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi province 710127, PR China. Electronic address: lijianli@nwu.edu.cn.

PMID: 33080512
DOI: 10.1016/j.saa.2020.119041

Abstract

Glutathione (GSH) is one of the most essential bio-thiols to maintain the redox balance of organisms which is strongly associated with many physiological processes. Detecting the concentration and mapping the distribution of GSH in the living system is significant to study many related diseases. In this work, we have successfully constructed an ICT-based model to guide the design and synthesis of GSH specific fluorescent probe CF1. A serials spectroscopy test demonstrated that the response of CF1 towards GSH owned large stokes shift (~167 nm) and an excellent linear relationship (0-120 μM, R² = 0.9961). Furthermore, CF1 was successfully applied to image endogenous GSH in different cell lines with high sensitivity. This work is instructive for the oriented synthesis of ICT-based functional fluorescent probe and the further visualization of intracellular targets in the living system.

Keywords: Cells imaging; Fluorescent probe; Glutathione; ICT; Isophorone; Theoretical calculation.

MeSH terms

Cell Line
Fluorescent Dyes*
Glutathione*

Substances

Fluorescent Dyes
Glutathione