Identification and Characterization of a Novel FosA7 Member from Fosfomycin-Resistant Escherichia coli Clinical Isolates from Canadian Hospitals

Antimicrob Agents Chemother. 2020 Dec 16;65(1):e00865-20. doi: 10.1128/AAC.00865-20. Print 2020 Dec 16.

Abstract

Here, we characterize the fosA genes from three Escherichia coli clinical isolates recovered from Canadian patients. Each fosA sequence was individually overexpressed in E. coli BW25113, and antimicrobial susceptibility testing was performed to assess their role in fosfomycin resistance. The findings from this study identify and functionally characterize FosA3, FosA8, and novel FosA7 members and highlight the importance of phenotypic characterization of fosA genes.

Keywords: Escherichia coli; beta-lactamase; fosA7; fosfomycin; novel fosA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Canada
  • Drug Resistance, Bacterial / genetics
  • Escherichia coli / genetics
  • Escherichia coli Infections* / drug therapy
  • Fosfomycin* / pharmacology
  • Hospitals
  • Humans
  • Microbial Sensitivity Tests
  • Plasmids
  • beta-Lactamases / genetics

Substances

  • Anti-Bacterial Agents
  • Fosfomycin
  • beta-Lactamases