A novel electrochemical immunosensor for hepatitis B surface antigen based on Fe3O4 nanoflowers and heterogeneous chain reaction signal amplification strategy

Xiaoyan Li; Tianchen Liu; Yun Zhang; Xiao Ni; M Nur Hossain; Xiaojun Chen; He Huang; Heinz-Bernhard Kraatz

doi:10.1016/j.talanta.2020.121459

A novel electrochemical immunosensor for hepatitis B surface antigen based on Fe₃O₄ nanoflowers and heterogeneous chain reaction signal amplification strategy

Talanta. 2021 Jan 1:221:121459. doi: 10.1016/j.talanta.2020.121459. Epub 2020 Jul 31.

Authors

Xiaoyan Li¹, Tianchen Liu², Yun Zhang³, Xiao Ni⁴, M Nur Hossain⁵, Xiaojun Chen⁶, He Huang⁷, Heinz-Bernhard Kraatz⁸

Affiliations

¹ School of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, PR China; College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing, 211816, PR China.
² Nanjing Foreign Language School, Nanjing, 210018, PR China.
³ Zhongda Hospital, Affiliated to Southeast University, Nanjing, 210009, PR China.
⁴ College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing, 211816, PR China.
⁵ Department of Physical and Environmental Sciences, University of Toronto Scarborough, 1065, Military Trail, Toronto, ON M1C 1A4, Canada.
⁶ College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing, 211816, PR China. Electronic address: chenxj@njtech.edu.cn.
⁷ School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing, 211816, PR China; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210023, PR China. Electronic address: huangh@njtech.edu.cn.
⁸ Department of Physical and Environmental Sciences, University of Toronto Scarborough, 1065, Military Trail, Toronto, ON M1C 1A4, Canada. Electronic address: bernie.kraatz@utoronto.ca.

PMID: 33076081
DOI: 10.1016/j.talanta.2020.121459

Abstract

Herein, a novel sandwich-type electrochemical immunosensor was fabricated based on Fe₃O₄ nanoflowers (Fe₃O₄ NFs) and heterogeneous chain reaction (HCR) signal amplification strategy for the sensitive detection of hepatitis B surface antigen (HBsAg). The aldehyde-functionalized Fe₃O₄ NFs are used as a supporting matrix to immobilize the hepatitis B surface antibody 1 (HBsAb1). The biotin-modified single-strand DNA (biotin-S0) was connected onto the biotin-HBsAb2 via linkage of streptavidin (SA), followed by addition of methylene blue (MB) modified single strand DNA1 (MB-S1) and DNA2 (MB-S2) for HCR signal amplification. The designed immunosensor exhibited a detection linear range of 0.5 pg mL^-1-0.25 ng mL^-1 and a low detection limit of 0.16 pg mL^-1, with excellent stability, selectivity and reproducibility. Furthermore, HBsAg is detected in the serum samples with a stable and fast response, indicating that the proposed immunosensor has a promising potential application in clinical analysis.

Keywords: Clinical diagnosis; Fe(3)O(4) nanoflower; Hepatitis B surface Antigen; Heterogeneous chain reaction; Sandwich-type.

MeSH terms

Biosensing Techniques*
Electrochemical Techniques
Gold
Hepatitis B Surface Antigens*
Immunoassay
Limit of Detection
Reproducibility of Results
Streptavidin

Substances

Hepatitis B Surface Antigens
Gold
Streptavidin