Microbial production of acetoin is eco-friendly and inexpensive when compared with its synthetic methods of production. In the present findings, bioproduction of acetoin in a typical bioreactor was discussed with a view to ascertain the seemingly comparative advantage of bioreactor system over shake flask, and more importantly, to confirm that corn steep liquor can indeed adequately be used as a replacement for other organic nitrogen sources. Taguchi design was statistically used to optimized the fermentation process which resulted in a 3-fold increase in molar yield (83%) corresponding to a six-fold increase in acetoin concentration (63.43 g/L), as compared to a similar study conducted in a shake flask. Although agitation rate was observed to be the most controlling, the bioreactor may underperform at agitation rate greater than 300 rpm. The optimum parameters for acetoin production in this study were 300 rpm agitation, 1.5 slpm aeration, 2 days fermentation time, and pH 6.5. The results show that the commercial production of acetoin can be envisioned using a biological approach that may be of economic advantage.
Keywords: Acetoin; Bioengineering; Biotechnology; Fermentation; Microbiology; Optimization; Taguchi design.
© 2020 The Authors.