Tyrosinase (TYR) is a crucial enzyme in melanin metabolism and catecholamine production, its abnormal overexpression is closely associated with many human diseases involving melanoma cancer, vitiligo, Parkinson's disease and so on. Herein, a dual-signal fluorescence sensing system for monitoring TYR activity is constructed depending on the transformation of blue-green fluorescence emission of copolymer. The developed sensing system is based on TYR catalyzing the hydroxylation of mono-phenol to o-diphenol and the conversion of fluorescence copolymer (FCP) blue emission (430 nm) and green emission (535 nm) in the presence of PEI. In the system, both blue and green emission exhibit a high selectivity and sensitivity (S/B up to 300 and 30 for blue and green emission, respectively) toward TYR in the range from 0.5 to 2.5 U/mL with the detection limit of 0.002 U/mL and 0.06 U/mL, respectively. Additionally, this assay is used to detect TYR in human serum with excellent recovery even at 30% human serum concentrations. Furthermore, it still has been successfully applied to TYR inhibitor screening by taking kojic acid as a model. We believe that our developed sensor has great potential application in TYR-associated disease diagnosis and treatment and drug discovery.
Keywords: Copolymer; Dual-signal; Fluorescence transformation; Levodopa; Tyrosinase.
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