Abnormal characteristic "round bottom flask" shape volume-based scattergram as a trigger to suspect persistent polyclonal B-cell lymphocytosis

Abstract

Background and aims: The diagnosis of persistent polyclonal B-cell lymphocytosis (PPBL) is often challenging because of the lack of features and the overlap with the peripheral expression of splenic marginal zone lymphomas (SMZL). To obtain new clues for PPBL detection and diagnosis, all data provided by the DxH 800 analyzer (including scatter and cell population data (CPD)) was exploited and combined using a machine learning (ML) approach.

Materials and methods: A total 211 samples from 101 normal controls and 110 patients (PPBL and SMZL) were assessed. Age, gender, full blood count, CPD, scatter, flags and CellaVision differentials were also considered. A ML model was built for true classification purposes.

Results: PPBL and SMZL shared increased absolute lymphoid counts, atypical lymphoid flag presence and CPD values (8 out of 14). A typical "round-bottom-flask" shape scattergram was described for the first time for PPBL which was also present in 51.4% of SMZL cases. The developed ML model render a global classification accuracy of 93.4%, allowing the detection of all pathological cases, with mean misclassification rates of 12% among PPBL and SMZL.

Conclusion: Our ML model represents a new unbiased tool than can be widely applied in the laboratory as an aid for detection of PPBL.

Keywords: Cell population data; Lymphocytosis; Machine learning algorithms; Persistent polyclonal B-cell lymphocytosis.