Objective: To study the difference of long non coding RNA (lncRNA) expression profile in bone marrow specimens of children with acute leukemia (AL) and other hematological disease children with normal bone marrows as controls, to screen the lncRNA related with childhood hematological diseases, and to explore the expression of lncRNA AC002454.1 and its clinical significance in AL children.
Methods: The microarray gene chip technology was used to statistically analyze the lncRNA in bone marrow cells of newly diagnose AL children and control children. Ninty-seren differentially expressed lncRNAs were selected. The bone marrow specimens of ALL children (21 cases), AML children (22 cases) and control children (21 cases) were verified and compared by using qRT-PCR; then the lncRNA with maximum differential expression-lncRNA AC002454.1 was selected and used to analyze the relation of relative expression level with clinical indicators.
Results: The microarray gene chip detection showed that 1 884 differentially expressed lncRNA were found in ALL children, and 4 289 differentically expressed lncRNA were found in AML children. The results confirming these differentically expressed lncRNA by qRT-PCR showed that 9 lncRNA expression were significantly up-regulated in ALL children, and 12 lncRNA expression were significantly up-regulated in AML children. Among these up-regulated lncRNA, the difference of AC002454.1 expression was most significant in ALL and AML children (P＜0.05, P＜0.01). The detection showed that there was a significant difference, in AC002454.1 relative expression level of newly diagnosed T-ALL and B-ALL children (P＜0.01), moreover, this difference also was found in ALL and AML children (P＜0.05). The detection analysis showed that there was no statistical difference in AC002454.1 relative expression level among the different sex, age, WBC count at initial diagnosis, chromosome, fusion gene, and risk stratification (P＞0.05 for all).
Conclusion: The lncRNA expression profile of AL children has been gained by using the lncRNA microarray gene chip technicology. AC002454.1 the significantly high expression exist in AL children, which relates with immunotyping and prognosis of AL children in a certain degree.
题目: 长链非编码RNA AC002454.1在急性白血病患儿中的表达及临床意义.
目的: 研究长链非编码RNA（long non-coding RNA, lncRNA）在急性白血病（acute leukemia, AL）患儿和骨髓正常的其它血液病患儿（作为对照）标本中的表达谱的差异, 筛选与AL患儿相关的lncRNA, 探讨lncRNA AC002454.1在AL患儿中的表达及临床意义.
方法: 采用Microarray基因芯片技术对初诊AL儿童和对照儿童骨髓细胞的lncRNA进行统计分析。选取差异表达显著的lncRNA 97个, 对急性淋巴细胞白血病（acute lymphoblastic leukemia, ALL）患儿（21例）、急性髓细胞白血病（acute myelocytic leukemia, AML）患儿（22例）和对照儿童（21例）样本采用实时荧光定量PCR（qRT-PCR）技术进行验证并比较。并选择其中差异最大的lncRNA AC002454.1分析其相对表达量与临床指标的关系.
结果: 经Microarray基因芯片检测, ALL患儿差异表达的lncRNA 1 884个、 AML患儿差异表达的lncRNA 4 289个。采用qRT-PCR对异常表达lncRNA进行验证的结果显示, ALL组中表达显著上调9例、AML组中表达显著上调12例, 其中AC002454.1在ALL、AML患儿表达差异最显著（P＜0.05, P＜0.01）。AC002454.1的相对表达量在初诊ALL免疫分型T系与B系中有显著差异（P＜0.01）、ALL和 AML 组有显著差异（P＜0.05）, AC002454.1相对表达量在患儿性别、年龄、初诊时WBC计数、染色体、融合基因、危 险度分层之间无显著差异（P值均＞0.05）.
结论: 用长链非编码RNA芯片分析获得了AL患儿的lncRNA表达谱, AC002454.1在AL患儿中异常高表达, 其表达量与AL患儿的免疫分型及判断患者预后有一定相关性.