Use of kinetic data to model potential antioxidant activity: Radical scavenging capacity of Australian Eucalyptus honeys

Soheila Beiranvand; Ashley Williams; Symsia Long; Peter R Brooks; Fraser D Russell

doi:10.1016/j.foodchem.2020.128332

Use of kinetic data to model potential antioxidant activity: Radical scavenging capacity of Australian Eucalyptus honeys

Food Chem. 2021 Apr 16:342:128332. doi: 10.1016/j.foodchem.2020.128332. Epub 2020 Oct 8.

Authors

Soheila Beiranvand¹, Ashley Williams², Symsia Long³, Peter R Brooks⁴, Fraser D Russell⁵

Affiliations

¹ GeneCology Research Centre, University of the Sunshine Coast, Qld, Australia; School of Health and Sport Sciences, University of the Sunshine Coast, Qld, Australia; CRC for Honey Bee Products Ltd., 128 Yanchep Beach Road, Yanchep, WA, Australia. Electronic address: Soheila.Beiranvand@research.usc.edu.au.
² GeneCology Research Centre, University of the Sunshine Coast, Qld, Australia; School of Health and Sport Sciences, University of the Sunshine Coast, Qld, Australia.
³ GeneCology Research Centre, University of the Sunshine Coast, Qld, Australia; School of Health and Sport Sciences, University of the Sunshine Coast, Qld, Australia. Electronic address: l_s097@student.usc.edu.au.
⁴ GeneCology Research Centre, University of the Sunshine Coast, Qld, Australia; School of Science and Engineering, University of the Sunshine Coast, Qld, Australia; CRC for Honey Bee Products Ltd., 128 Yanchep Beach Road, Yanchep, WA, Australia. Electronic address: PBrooks@usc.edu.au.
⁵ GeneCology Research Centre, University of the Sunshine Coast, Qld, Australia; School of Health and Sport Sciences, University of the Sunshine Coast, Qld, Australia; CRC for Honey Bee Products Ltd., 128 Yanchep Beach Road, Yanchep, WA, Australia. Electronic address: frussell@usc.edu.au.

PMID: 33067037
DOI: 10.1016/j.foodchem.2020.128332

Abstract

Antioxidant activity of honeys may be beneficial in wound healing processes by protecting cells against lipid oxidation. The DPPH assay assesses the efficacy of antioxidant molecules to reduce DPPH^• to DPPHH. Studies determining EC₅₀ are limited by single time-point determinations of antioxidant effect and can miss vital information about the rate of antioxidant response. Acquisition of kinetic data allows determination of the radical scavenging capacity (RSC) of honeys. The purpose of this study was to determine the RSC of 53 honeys from 16 species of Australian Eucalyptus trees and four samples of New Zealand manuka (Leptospermum scoparium) honey. Whereas honeys could not be differentiated based on EC₅₀ values, significant differences were observed for RSC, supporting collection of kinetic data for honey analysis. The greatest RSC was observed for New Zealand manuka (4.6 ± 0.3 × 10^-5 mg.mL^-1.min^-1), grey ironbark (E. paniculate; 3.4 ± 0.2 × 10^-5 mg.mL^-1.min^-1) and river red gum honeys (E. camaldulensis; 3.2 ± 0.2 × 10^-5 mg.mL^-1.min^-1).

Keywords: Antioxidant activity; Eucalyptus; Honey; Radical scavenging capacity.

MeSH terms

Australia
Eucalyptus / chemistry*
Free Radical Scavengers / chemistry
Free Radical Scavengers / pharmacology*
Honey* / analysis
Kinetics
Leptospermum / chemistry
Models, Biological*

Substances

Free Radical Scavengers