Freshwater mud-eel, Ophichthys cuchia is nocturnal, carnivorous and economically important fish, yet its digestive physiology is unknown. We therefore studied the gastrointestinal (GI) tract of O. cuchia using morphological, osteological, histological and histochemical approach to understand how the structural adequacy of GI tract helps in acquisition of bottom and mud-dwelling prey and supports utilization of uncommon food resources. Morphologically the GI tract showed typical features of carnivorous fishes in the form of sub-terminal mouth, short muscular esophagus, expendable stomach, short intestine and rectum. Osteological investigation clearly showed that the specialized arrangement of teeth in the oral cavity and pharyngeal region helps in digging and manipulation of bottom and mud-dwelling prey. Longitudinal mucosal folds, stratified squamous epithelium with numerous goblet cells of esophagus protect mucosa from mechanical harm and also allow easy transit of prey into the stomach. Large-sized rugae of stomach are complemented with numerous gastric glands which together increase the transit time of carnivorous food stuff and ensure its complete digestion. The pylorus specifically allows only digested and finely ground food stuff into the intestine and thus avoids the intestinal blockage due to entry of undigested food. The enterocytes and goblet cells of intestine are absorptive and lubricative in their functions respectively. A significant increase in the number of goblet cells and thickness of muscularis from the intestine to the rectum was evident which facilitates the easier transit of food, protection for the epithelium from abrasion, and helps in defecation. Histochemistry revealed that the mucins are secreted throughout the GI tract and thus catalyze faster digestion of carnivorous food stuff, providing protection to mucosal membrane from abrasion, acts as a co-factor to support digestion, absorption of proteins, ions, fluids and helps in defecation. The outcome of this study clearly supports the notion that structural adaptations in the digestive tract of fishes can be effectively used as a blueprint to understand why and how particular fish species feed and use unique food. Additionally, the structural and functional adequacy of the digestive tract helps the fishes to acquire and utilize novel habitats and food resources. The results presented in this study will serve as a reference point for future studies, which focus primarily on understanding the evolution of carnivory in Synbranchids.
Keywords: Gastro-intestinal tract; Goblet cells; Osteology; Pharyngeal teeth; Synbranchids.
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